Journal
/
Biochemistry
/
Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts

English

Automatically Generated

8,025 Views

•

06:31 min

•

September 27, 2018

DOI:

10.3791/58240-v

DOI:
10.3791/58240-v

•

06:31 min
September 27, 2018

•

7 Views
, , , ,
1Department of Biophysics,University of Michigan, Ann Arbor, 2Department of Chemistry,University of Michigan, Ann Arbor, 3Department of Computer Science,Wayne State University, 4Department of Physics,University of Michigan, Ann Arbor

Summary

Automatically generated

We present a method for the generation of in vitro self-sustained mitotic oscillations at the single-cell level by encapsulating egg extracts of Xenopus laevis in water-in-oil microemulsions.

Explore More Videos

Cell-cycle OscillationsXenopus Egg ExtractsMicroemulsionsCell-free SystemMitotic NetworkCell Cycle ClockHigh-throughputQuantitative ManipulationCell ActivationCell LysisCell Extract Preparation

Read Article

Cite this Article

Guan, Y., Wang, S., Jin, M., Xu, H., Yang, Q. Reconstitution of Cell-cycle Oscillations in Microemulsions of Cell-free Xenopus Egg Extracts. J. Vis. Exp. (139), e58240, doi:10.3791/58240 (2018).

Download .ris file

Copy

Share Video

.

Copy