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T7 bakteriyofaj Biopanning üzerinden kantitatif PCR
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative PCR of T7 Bacteriophage from Biopanning
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T7 bakteriyofaj Biopanning üzerinden kantitatif PCR

Türkçe

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10,451 Views

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05:42 min

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September 27, 2018

DOI:

10.3791/58165-v

DOI:
10.3791/58165-v

•

05:42 min
September 27, 2018

•

6 Views
, , , ,
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy,University of Texas at Austin

Summary

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T7 bakteriyofaj numaralandırmak için bir tekrarlanabilir, doğru ve zamanı verimli kantitatif PCR (qPCR) yöntemi burada açıklanmıştır. Protokol açıkça fajının genomik DNA hazırlık, PCR reaksiyon hazırlama, qPCR Bisiklet koşullarını ve qPCR veri analizi açıklar.

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Cite this Article

Peng, X., Leal, J., Mohanty, R., Soto, M., Ghosh, D. Quantitative PCR of T7 Bacteriophage from Biopanning. J. Vis. Exp. (139), e58165, doi:10.3791/58165 (2018).

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