Journal
/
Immunology and Infection
/
PCR quantitative du bactériophage T7 de criblage
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Quantitative PCR of T7 Bacteriophage from Biopanning
Please note that all translations are automatically generated. Click here for the English version.

PCR quantitative du bactériophage T7 de criblage

français

Automatically Generated

10,451 Views

•

05:42 min

•

September 27, 2018

DOI:

10.3791/58165-v

DOI:
10.3791/58165-v

•

05:42 min
September 27, 2018

•

6 Views
, , , ,
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy,University of Texas at Austin

Summary

Automatically generated

Reproductible, précise et temps efficace quantitative PCR (qPCR) décrit une méthode pour énumérer bactériophage T7 est ici. Le protocole décrit clairement la préparation d’ADN génomique de phage, préparation de réaction PCR, qPCR conditions cyclisme et analyse de données de qPCR.

Explore More Videos

Quantitative PCRT7 BacteriophageBiopanningVirologyMicrobiologyPhage EnumerationPhage DiagnosticsPhage TherapeuticsPhage DisplayPrimer DesignReference DNASerial DilutionGenome CopiesDNase TreatmentHeat InactivationQPCR Premix96-well PlateFluorescencePhotobleaching

Read Article

Cite this Article

Peng, X., Leal, J., Mohanty, R., Soto, M., Ghosh, D. Quantitative PCR of T7 Bacteriophage from Biopanning. J. Vis. Exp. (139), e58165, doi:10.3791/58165 (2018).

Download .ris file

Copy

Share Video

.

Copy