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Quantitative PCR of T7 Bacteriophage from Biopanning
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative PCR of T7 Bacteriophage from Biopanning

Quantitative PCR of T7 Bacteriophage from Biopanning

DOI:
10.3791/58165-v

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05:42 min

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September 27, 2018

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, , , ,
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy,University of Texas at Austin

Chapters

  • 00:04Title
  • 00:43Prepare Standards for Quantification of T7 Phage Genomic DNA
  • 01:30Pre-treatment of Phage Samples Before qPCR Reaction Preparation
  • 02:29Prepare qPCR Reactions and qPCR Cycling Conditions
  • 03:31Results: Quantitative PCR of T7 Bacteriophage
  • 04:49Conclusion

Summary

Automatic Translation

Automatic Translation

A reproducible, accurate, and time efficient quantitative PCR (qPCR) method to enumerate T7 bacteriophage is described here. The protocol clearly describes phage genomic DNA preparation, PCR reaction preparation, qPCR cycling conditions, and qPCR data analysis.

Tags

Quantitative PCRT7 BacteriophageBiopanningVirologyMicrobiologyPhage EnumerationPhage DiagnosticsPhage TherapeuticsPhage DisplayPrimer DesignReference DNASerial DilutionGenome CopiesDNase TreatmentHeat InactivationQPCR Premix96-well PlateFluorescencePhotobleaching

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