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Quantitative PCR of T7 Bacteriophage from Biopanning
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative PCR of T7 Bacteriophage from Biopanning

Quantitative PCR of T7 Bacteriophage from Biopanning

English

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10,451 Views

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05:42 min

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September 27, 2018

DOI:

10.3791/58165-v

DOI:
10.3791/58165-v

•

05:42 min
September 27, 2018

•

6 Views
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1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy,University of Texas at Austin

Summary

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A reproducible, accurate, and time efficient quantitative PCR (qPCR) method to enumerate T7 bacteriophage is described here. The protocol clearly describes phage genomic DNA preparation, PCR reaction preparation, qPCR cycling conditions, and qPCR data analysis.

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Peng, X., Leal, J., Mohanty, R., Soto, M., Ghosh, D. Quantitative PCR of T7 Bacteriophage from Biopanning. J. Vis. Exp. (139), e58165, doi:10.3791/58165 (2018).

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