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Quantitative PCR von Bakteriophagen T7 aus Biopanning
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative PCR of T7 Bacteriophage from Biopanning
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Quantitative PCR von Bakteriophagen T7 aus Biopanning

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10,451 Views

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05:42 min

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September 27, 2018

DOI:

10.3791/58165-v

DOI:
10.3791/58165-v

•

05:42 min
September 27, 2018

•

6 Views
, , , ,
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy,University of Texas at Austin

Summary

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Eine reproduzierbare und genaue Zeit effizientere quantitative PCR (qPCR) Methode aufzuzählenden T7 Bakteriophagen wird hier beschrieben. Das Protokoll beschreibt klar Phagen genomische DNA-Vorbereitung, PCR Reaktion Vorbereitung, qPCR Radsport Bedingungen und qPCR Datenanalyse.

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Peng, X., Leal, J., Mohanty, R., Soto, M., Ghosh, D. Quantitative PCR of T7 Bacteriophage from Biopanning. J. Vis. Exp. (139), e58165, doi:10.3791/58165 (2018).

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