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人間でタグ内因性のタンパク質誘導多能性幹細胞 CRISPR/Cas9 を使用して
JoVE Journal
Genetics
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JoVE Journal Genetics
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
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人間でタグ内因性のタンパク質誘導多能性幹細胞 CRISPR/Cas9 を使用して

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25,128 Views

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14:48 min

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August 25, 2018

DOI:

10.3791/58130-v

DOI:
10.3791/58130-v

•

14:48 min
August 25, 2018

•

36 Views
, , , , ,
1Allen Institute for Cell Science

Summary

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タグ内因のプロトコル CRISPR/Cas9 を用いたひと誘導多能性幹細胞の蛍光札が付いている蛋白質の表現は、ここで説明。推定編集されたセル、蛍光活性化細胞選別濃縮し、クローン細胞を生成します。

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Cite this Article

Haupt, A., Grancharova, T., Arakaki, J., Fuqua, M. A., Roberts, B., Gunawardane, R. N. Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. J. Vis. Exp. (138), e58130, doi:10.3791/58130 (2018).

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