ジャーナル
/
/
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
JoVE Journal
遺伝学
Author Produced
This content is Free Access.
JoVE Journal 遺伝学
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

25,131 Views

14:48 min

August 25, 2018

DOI:

14:48 min
August 25, 2018

36 Views
, , , , ,

概要

Automatically generated

Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

Read Article