This content is Free Access.
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
25,061 Views
•
14:48 min
•
August 25, 2018
DOI:
Summary
Automatically generated
Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.
Read Article
Cite this Article
Haupt, A., Grancharova, T., Arakaki, J., Fuqua, M. A., Roberts, B., Gunawardane, R. N. Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. J. Vis. Exp. (138), e58130, doi:10.3791/58130 (2018).
Copy