Journal
/
Genética
/
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
JoVE Journal
Genética
Author Produced
This content is Free Access.
JoVE Journal Genética
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

25,061 Views

•

14:48 min

•

August 25, 2018

DOI:

10.3791/58130-v

DOI:
10.3791/58130-v

•

14:48 min
August 25, 2018

•

36 Views
, , , , ,
1Allen Institute for Cell Science

Summary

Automatically generated

Described here is a protocol for tagging endogenously expressed proteins with fluorescent tags in human induced pluripotent stem cells using CRISPR/Cas9. Putatively edited cells are enriched by fluorescence activated cell sorting and clonal cell lines are generated.

Explore More Videos

CRISPR/Cas9Endogenous Protein TaggingHuman Induced Pluripotent Stem CellsRibonucleoproteinFluorescent TagFACSClonal Cell LinesGene EditingLive Cell ImagingCellular StructuresCellular Processes

Read Article

Cite this Article

Haupt, A., Grancharova, T., Arakaki, J., Fuqua, M. A., Roberts, B., Gunawardane, R. N. Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. J. Vis. Exp. (138), e58130, doi:10.3791/58130 (2018).

Download .ris file

Copy

Share Video

.

Copy