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Journal
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Immunology and Infection
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高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
Please note that all translations are automatically generated.
Click here for the English version.
高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究
DOI:
10.3791/58074-v
•
07:22 min
•
August 14, 2018
•
Anthony M. Esposito
2
,
Alexandra Y. Soare
,
Foramben Patel
,
Namita Satija
,
Benjamin K. Chen
,
Talia H. Swartz
1
Division of Infectious Diseases, Department of Medicine
,
Icahn School of Medicine at Mount Sinai, Immunology Institute
,
2
Department of Biology
,
New Jersey City University
Chapters
00:04
Title
00:54
Preparation of Target Cells for Cell-to-Cell Virus Transmission
02:11
Preparation of Donor Cells
03:56
Co-culture
05:06
Fluorescence-activated Cell Sorting
05:35
Results: Identification of a Novel HIV-1 Viral Membrane Fusion Inhibitor
06:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
通过
流式细胞术或荧光显微镜检测绿色荧光蛋白的表达, 我们描述了以细胞为基础的 HIV-1 融合报告。它可以用来测试病毒进入的抑制剂 (特别是在融合步骤) 在无细胞和细胞对细胞感染系统。
Tags
Cre-Lox
Viral Membrane Fusion
HIV-1
Drug Discovery
High-throughput Screening
HIV Fusion Inhibitors
Biosafety
Jurkat RG Reporter Cells
Puromycin
Cell-to-cell Virus Transmission
Gag-iCre DNA
Electroporation
Ficoll
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