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SiRNA Nanoparticle-mediada-silenciamento no coração de Zebrafish adulto
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Developmental Biology
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JoVE Journal Developmental Biology
Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

SiRNA Nanoparticle-mediada-silenciamento no coração de Zebrafish adulto

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7,953 Views

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09:12 min

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July 29, 2018

DOI:

10.3791/58054-v

DOI:
10.3791/58054-v

•

09:12 min
July 29, 2018

•

1 Views
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1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine,Peking University, 2Department of Biomedical Engineering, College of Engineering,Peking University

Summary

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Continua a ser um grande desafio para desenvolver condicional gene nocaute ou eficaz knockdown do gene-em órgãos adultos do zebrafish. Aqui nós relatamos um protocolo para desempenho siRNA nanoparticle-mediada silenciamento no coração de adultos do zebrafish, proporcionando assim um novo método de perda-de-função para estudar órgãos adultos no zebrafish e outros organismos-modelo.

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Cite this Article

Xiao, C., Wang, F., Hou, J., Zhu, X., Luo, Y., Xiong, J. Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart. J. Vis. Exp. (137), e58054, doi:10.3791/58054 (2018).

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