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Nanoparticelle-mediata siRNA silenziamento genico nel cuore di Zebrafish adulto
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Developmental Biology
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JoVE Journal Developmental Biology
Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
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Nanoparticelle-mediata siRNA silenziamento genico nel cuore di Zebrafish adulto

italiano

Automatically Generated

7,953 Views

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09:12 min

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July 29, 2018

DOI:

10.3791/58054-v

DOI:
10.3791/58054-v

•

09:12 min
July 29, 2018

•

1 Views
, , , , ,
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine,Peking University, 2Department of Biomedical Engineering, College of Engineering,Peking University

Summary

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Rimane una grande sfida di sviluppare condizionale gene knock-out o efficace gene-atterramento in organi di zebrafish adulto. Qui segnaliamo un protocollo per la performante siRNA nanoparticella-mediata silenziamento genico nel cuore adulto zebrafish, fornendo così un nuovo metodo di perdita di funzione per lo studio di organi adulti in zebrafish e altri organismi di modello.

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Cite this Article

Xiao, C., Wang, F., Hou, J., Zhu, X., Luo, Y., Xiong, J. Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart. J. Vis. Exp. (137), e58054, doi:10.3791/58054 (2018).

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