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Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

7,956 Views

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09:12 min

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July 29, 2018

DOI:

10.3791/58054-v

DOI:
10.3791/58054-v

•

09:12 min
July 29, 2018

•

1 Views
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1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine,Peking University, 2Department of Biomedical Engineering, College of Engineering,Peking University

Summary

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It remains a major challenge to develop conditional gene-knockout or effective gene-knockdown in adult zebrafish organs. Here we report a protocol for performing nanoparticle-mediated siRNA gene-silencing in adult zebrafish heart, thus providing a new loss-of-function method for studying adult organs in zebrafish and other model organisms.

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Cite this Article

Xiao, C., Wang, F., Hou, J., Zhu, X., Luo, Y., Xiong, J. Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart. J. Vis. Exp. (137), e58054, doi:10.3791/58054 (2018).

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