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分離してチラコイド脂質リポソームの珪藻峰 Meneghinianaから光捕集アンテナを組み込む
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
DOI:

11:28 min

August 28, 2018

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Chapters

  • 00:04Title
  • 00:34Thylakoid Isolation from C. meneghiniana Cells
  • 03:13Separation and Concentration of FCP Complexes
  • 05:22Preparation of Liposomes and Incorporation of FCP
  • 07:18Incorporation of FCP Complexes and Removal of Detergents and Aggregates
  • 09:59Results: Spectral Features of FCP-liposomes in Response to Ion Gradients
  • 11:07Conclusion

Summary

Automatic Translation

フコキサンチン クロロフィル a/c 結合タンパク質 (FCP) 珪藻を分離し、天然の脂質組成のイオン組成変化の励起エネルギー移動を研究するリポソームにそれらを組み込むのためのプロトコルを紹介します。

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