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Isolando e incorporando luce-raccolta di antenne da diatomea Cyclotella Meneghiniana in liposomi con i lipidi Thylakoid
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
DOI:

11:28 min

August 28, 2018

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Chapters

  • 00:04Title
  • 00:34Thylakoid Isolation from C. meneghiniana Cells
  • 03:13Separation and Concentration of FCP Complexes
  • 05:22Preparation of Liposomes and Incorporation of FCP
  • 07:18Incorporation of FCP Complexes and Removal of Detergents and Aggregates
  • 09:59Results: Spectral Features of FCP-liposomes in Response to Ion Gradients
  • 11:07Conclusion

Summary

Automatic Translation

Qui, presentiamo un protocollo per isolare le proteine che legano fucoxantina clorofilla a/c (FCP) da diatomee e incorporarli nei liposomi con composizioni lipidiche naturali per studiare il trasferimento di energia di eccitazione su cambiamenti della composizione dello ione.

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