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类囊脂质体中硅藻Cyclotella Meneghiniana中的光捕获天线的分离与融合
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
DOI:

11:28 min

August 28, 2018

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Chapters

  • 00:04Title
  • 00:34Thylakoid Isolation from C. meneghiniana Cells
  • 03:13Separation and Concentration of FCP Complexes
  • 05:22Preparation of Liposomes and Incorporation of FCP
  • 07:18Incorporation of FCP Complexes and Removal of Detergents and Aggregates
  • 09:59Results: Spectral Features of FCP-liposomes in Response to Ion Gradients
  • 11:07Conclusion

Summary

Automatic Translation

在这里, 我们提出了一种从硅藻中分离 fucoxanthin 叶绿素 a/c 结合蛋白 (FCP) 的协议, 并将其纳入脂质体和天然脂质成分, 研究了离子成分变化时的激发能量传递。

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