Journal
/
/
Isolere og indarbejde lys-høst antenner fra Diatom Cyclotella Meneghiniana i Liposomer med tylakoid lipider
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Isolating and Incorporating Light-Harvesting Antennas from Diatom Cyclotella Meneghiniana in Liposomes with Thylakoid Lipids
DOI:

11:28 min

August 28, 2018

, ,

Chapters

  • 00:04Title
  • 00:34Thylakoid Isolation from C. meneghiniana Cells
  • 03:13Separation and Concentration of FCP Complexes
  • 05:22Preparation of Liposomes and Incorporation of FCP
  • 07:18Incorporation of FCP Complexes and Removal of Detergents and Aggregates
  • 09:59Results: Spectral Features of FCP-liposomes in Response to Ion Gradients
  • 11:07Conclusion

Summary

Automatic Translation

Vi præsenterer her, en protokol for at isolere fucoxanthin klorofyl a/c bindende proteiner (FCP) fra kiselalger og indarbejde dem i Liposomer med naturlige lipid kompositioner at studere excitation energioverførsel ved ion sammensætning ændringer.

Related Videos

Read Article