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Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

10,701 Views

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10:05 min

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September 30, 2018

DOI:

10.3791/58000-v

DOI:
10.3791/58000-v

•

10:05 min
September 30, 2018

•

23 Views
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1College of Life Sciences, Peking-Tsinghua Center for Life Sciences,Peking University, 2Academy for Advanced Interdisciplinary Studies,Peking University, 3PKU-Tsinghua-NIBS Graduate Program,Peking University

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We describe a method for the isolation of endocrine cells from embryonic, neonatal and postnatal pancreases followed by single-cell RNA sequencing. This method allows analyses of pancreatic endocrine lineage development, cell heterogeneity and transcriptomic dynamics.

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Single-cell TranscriptomicsPancreatic Endocrine CellsLineage DifferentiationMaturationRegenerationDiabetesPancreatic Endocrine DiseasesCollagenase PerfusionBile DuctMouse Pancreas

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Li, L., Yu, X., Zhang, Y., Feng, Y., Qiu, W., Xu, C. Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells. J. Vis. Exp. (139), e58000, doi:10.3791/58000 (2018).

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