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A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue
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Behavior
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JoVE Journal Behavior
A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue

A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue

English

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6,142 Views

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08:14 min

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August 15, 2018

DOI:

10.3791/57909-v

DOI:
10.3791/57909-v

•

08:14 min
August 15, 2018

•

2 Views
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1Department of Pharmacology,Louisiana State University Health Sciences Center, 2Department of Surgery,Louisiana State University Health Sciences Center

Summary

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White adipose tissue (WAT) has critical deficiencies in its current primary culture models, hindering pharmacological development and metabolic studies. Here, we present a protocol to produce an adipose microphysiological system by sandwiching WAT between sheets of stromal cells. This construct provides a stable and adaptable platform for primary WAT culture.

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Cite this Article

Scahill, S. D., Hunt, M., Rogers, C. L., Lau, F. H. A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue. J. Vis. Exp. (138), e57909, doi:10.3791/57909 (2018).

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