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A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue
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August 15, 2018
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White adipose tissue (WAT) has critical deficiencies in its current primary culture models, hindering pharmacological development and metabolic studies. Here, we present a protocol to produce an adipose microphysiological system by sandwiching WAT between sheets of stromal cells. This construct provides a stable and adaptable platform for primary WAT culture.
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Scahill, S. D., Hunt, M., Rogers, C. L., Lau, F. H. A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue. J. Vis. Exp. (138), e57909, doi:10.3791/57909 (2018).
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