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A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue
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JoVE Journal Behavior
A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue

A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue

DOI:
10.3791/57909-v

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08:14 min

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August 15, 2018

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, , ,
1Department of Pharmacology,Louisiana State University Health Sciences Center, 2Department of Surgery,Louisiana State University Health Sciences Center

Chapters

  • 00:04Title
  • 00:34Sandwiching Cell Sheet Seeding and SWAT Supply Preparation
  • 02:02Gelatin Plunger Preparation
  • 03:40White Adipose Tissue Processing
  • 05:49Results: Representative SWAT Analyses
  • 07:23Conclusion

Summary

Automatic Translation

Automatic Translation

White adipose tissue (WAT) has critical deficiencies in its current primary culture models, hindering pharmacological development and metabolic studies. Here, we present a protocol to produce an adipose microphysiological system by sandwiching WAT between sheets of stromal cells. This construct provides a stable and adaptable platform for primary WAT culture.

Tags

Keywords: Microphysiologic PlatformHuman FatAdipose BiologyAdipose Stromal Support CellsASCPNIPAAmGelatin SolutionSeeding ApparatusHBSSSodium Hydroxide

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