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HEK 細胞から Mitoribosome の急速な隔離
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Rapid Isolation of the Mitoribosome from HEK Cells
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HEK 細胞から Mitoribosome の急速な隔離

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10,318 Views

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09:33 min

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October 04, 2018

DOI:

10.3791/57877-v

DOI:
10.3791/57877-v

•

09:33 min
October 04, 2018

•

9 Views
, , ,
1Science for Life Laboratory, Department of Biochemistry and Biophysics,Stockholm University

Summary

Automatically generated

ミトコンドリアは、細菌や細胞質相手から乖離したリボソームを特化しました。HEK 細胞の彼らのネイティブのコンパートメントからの mitoribosomes の取得方法を紹介します。懸濁細胞からミトコンドリアの分離と mitoribosomes の結果として浄化メソッドが含まれます。

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Cite this Article

Aibara, S., Andréll, J., Singh, V., Amunts, A. Rapid Isolation of the Mitoribosome from HEK Cells. J. Vis. Exp. (140), e57877, doi:10.3791/57877 (2018).

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