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Rapid Isolation of the Mitoribosome from HEK Cells
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Rapid Isolation of the Mitoribosome from HEK Cells

Rapid Isolation of the Mitoribosome from HEK Cells

DOI:
10.3791/57877-v

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09:33 min

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October 04, 2018

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, , ,
1Science for Life Laboratory, Department of Biochemistry and Biophysics,Stockholm University

Capítulos

  • 00:04Título
  • 00:53Mitochondria Isolation
  • 05:15Mitoribosome Purification
  • 08:12Results: Isolated Mitoribosomes from HEK Cells
  • 09:10Conclusion

Summary

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Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.

Tags

MitoribosomeHEK CellsProtein SynthesisMitochondriaNitrogen CavitationCell LysisHigh-resolution StructureCancer TreatmentHEK293S CellsPBSMIB BufferSM4 BufferNitrogen Cavitation ChamberCell DebrisNucleiMiBSM BufferTeflon Glass Down Homogenizer

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