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Rapid Isolation of the Mitoribosome from HEK Cells
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October 04, 2018
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Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.
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Aibara, S., Andréll, J., Singh, V., Amunts, A. Rapid Isolation of the Mitoribosome from HEK Cells. J. Vis. Exp. (140), e57877, doi:10.3791/57877 (2018).
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