Journal
/
生物化学
/
Rapid Isolation of the Mitoribosome from HEK Cells
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Rapid Isolation of the Mitoribosome from HEK Cells

Rapid Isolation of the Mitoribosome from HEK Cells

DOI:
10.3791/57877-v

•

09:33 min

•

October 04, 2018

•

, , ,
1Science for Life Laboratory, Department of Biochemistry and Biophysics,Stockholm University

Chapters

  • 00:04Title
  • 00:53Mitochondria Isolation
  • 05:15Mitoribosome Purification
  • 08:12Results: Isolated Mitoribosomes from HEK Cells
  • 09:10Conclusion

Summary

自动翻译

自动翻译

Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.

Tags

MitoribosomeHEK CellsProtein SynthesisMitochondriaNitrogen CavitationCell LysisHigh-resolution StructureCancer TreatmentHEK293S CellsPBSMIB BufferSM4 BufferNitrogen Cavitation ChamberCell DebrisNucleiMiBSM BufferTeflon Glass Down Homogenizer

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei

Isolation of Tissue Extracellular Vesicles from the Liver

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Read Article