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Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts
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Immunology and Infection
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JoVE Journal Immunology and Infection
Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts
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Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

DOI:
10.3791/57870-v

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11:15 min

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September 01, 2018

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, , , , ,
1INSERM, CNRS, Université d’Angers, Université de Nantes,CRCINA, 2Immunotherapy, Graft, Oncology,LabEx IGO, 3Hopital de Nantes,Hotel Dieu

Chapters

  • 00:01Title
  • 01:37Preoperative Effector Cells Preparation
  • 03:50Stereotactic Injection: Surgical Procedure
  • 07:33Representative Results
  • 09:50Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the preparation and the stereotaxic administration of allogeneic human lymphocytes in immunodeficient mice carrying orthotopic human primary brain tumors. This study provides a proof-of-concept for both the feasibility and the antitumor efficacy of intrabrain-delivered cellular immunotherapies.

Tags

Glioblastoma MultiformeCellular ImmunotherapyCytotoxic Immune CellsOrthotopic XenograftsStereotactic InjectionGBM-reactive Effector CellsNSG Mouse ModelTumor MicroenvironmentAdoptive Cell Transfer

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