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Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts
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JoVE 杂志 免疫与感染
Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

DOI:
10.3791/57870-v

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11:15 min

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September 01, 2018

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, , , , ,
1INSERM, CNRS, Université d’Angers, Université de Nantes,CRCINA, 2Immunotherapy, Graft, Oncology,LabEx IGO, 3Hopital de Nantes,Hotel Dieu

Chapters

  • 00:01Title
  • 01:37Preoperative Effector Cells Preparation
  • 03:50Stereotactic Injection: Surgical Procedure
  • 07:33Representative Results
  • 09:50Conclusion

Summary

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Here, we present a protocol for the preparation and the stereotaxic administration of allogeneic human lymphocytes in immunodeficient mice carrying orthotopic human primary brain tumors. This study provides a proof-of-concept for both the feasibility and the antitumor efficacy of intrabrain-delivered cellular immunotherapies.

Tags

Glioblastoma MultiformeCellular ImmunotherapyCytotoxic Immune CellsOrthotopic XenograftsStereotactic InjectionGBM-reactive Effector CellsNSG Mouse ModelTumor MicroenvironmentAdoptive Cell Transfer

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