Rivista
/
Immunologia e infezioni
/
Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts
JoVE Journal
Immunologia e infezioni
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

DOI:
10.3791/57870-v

•

11:15 min

•

September 01, 2018

•

, , , , ,
1INSERM, CNRS, Université d’Angers, Université de Nantes,CRCINA, 2Immunotherapy, Graft, Oncology,LabEx IGO, 3Hopital de Nantes,Hotel Dieu

Capitoli

  • 00:01Titolo
  • 01:37Preoperative Effector Cells Preparation
  • 03:50Stereotactic Injection: Surgical Procedure
  • 07:33Representative Results
  • 09:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol for the preparation and the stereotaxic administration of allogeneic human lymphocytes in immunodeficient mice carrying orthotopic human primary brain tumors. This study provides a proof-of-concept for both the feasibility and the antitumor efficacy of intrabrain-delivered cellular immunotherapies.

Tags

Glioblastoma MultiformeCellular ImmunotherapyCytotoxic Immune CellsOrthotopic XenograftsStereotactic InjectionGBM-reactive Effector CellsNSG Mouse ModelTumor MicroenvironmentAdoptive Cell Transfer

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

Seven Steps to Stellate Cells

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors

Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells

Read Article