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January 10, 2019
DOI:
1Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology,Shizuoka University, 2Department of Bioscience, Graduated School of Science and Technology,Shizuoka University, 3Japan Collection of Microorganisms,RIKEN BioResource Research Center
Summary
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Con el objetivo de entender el comportamiento de los distintos elementos de ADN conjugativos bacterianos bajo condiciones diferentes, se describe un protocolo para la detección de diferencias en la frecuencia de conjugación, con alta resolución, para estimar cómo eficazmente la bacteria donante inicia verbal.
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Cite this Article
Shintani, M., Ohkuma, M., Kimbara, K. High-Resolution Comparison of Bacterial Conjugation Frequencies. J. Vis. Exp. (143), e57812, doi:10.3791/57812 (2019).
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