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High-Resolution Comparison of Bacterial Conjugation Frequencies
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January 10, 2019
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1Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology,Shizuoka University, 2Department of Bioscience, Graduated School of Science and Technology,Shizuoka University, 3Japan Collection of Microorganisms,RIKEN BioResource Research Center
Summary
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With an aim to understand the behaviors of various bacterial conjugative DNA elements under different conditions, we describe a protocol for detecting differences in conjugation frequency, with high resolution, to estimate how efficiently the donor bacterium initiates conjugation.
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Shintani, M., Ohkuma, M., Kimbara, K. High-Resolution Comparison of Bacterial Conjugation Frequencies. J. Vis. Exp. (143), e57812, doi:10.3791/57812 (2019).
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