With an aim to understand the behaviors of various bacterial conjugative DNA elements under different conditions, we describe a protocol for detecting differences in conjugation frequency, with high resolution, to estimate how efficiently the donor bacterium initiates conjugation.
Shintani, M., Ohkuma, M., Kimbara, K. High-Resolution Comparison of Bacterial Conjugation Frequencies. J. Vis. Exp. (143), e57812, doi:10.3791/57812 (2019).