需要订阅 JoVE 才能查看此. 登录或开始免费试用。
High-Resolution Comparison of Bacterial Conjugation Frequencies
10,434 Views
•
05:18 min
•
January 10, 2019
DOI:
1Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology,Shizuoka University, 2Department of Bioscience, Graduated School of Science and Technology,Shizuoka University, 3Japan Collection of Microorganisms,RIKEN BioResource Research Center
Summary
Automatically generated
With an aim to understand the behaviors of various bacterial conjugative DNA elements under different conditions, we describe a protocol for detecting differences in conjugation frequency, with high resolution, to estimate how efficiently the donor bacterium initiates conjugation.
Read Article
Cite this Article
Shintani, M., Ohkuma, M., Kimbara, K. High-Resolution Comparison of Bacterial Conjugation Frequencies. J. Vis. Exp. (143), e57812, doi:10.3791/57812 (2019).
Copy