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Definerede Xeno-fri og Feeder-fri kultur betingelser for den Generation af menneskelige iPSC-afledte Retinal Cell modeller
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
DOI:

11:29 min

September 06, 2018

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Chapters

  • 00:04Title
  • 01:04Generation and Maturation of Retinal Organoids
  • 02:50Generation and Amplification of Human iPSC-derived RPE (hiRPE) Cells
  • 05:17Cryopreservation of Retinal Organoids and hiRPE Cells
  • 07:09Thawing of Retinal Organoids and hiRPE Cells
  • 09:01Results: Generation of All Neural Retina Cell Types and RPE
  • 10:19Conclusion

Summary

Automatic Translation

Produktionen af specialiserede retinale celler fra pluripotente stamceller er et vendepunkt i udviklingen af stamcelle-baseret terapi for retinal sygdomme. Den nuværende papir beskriver en simpel metode til en effektiv generation af retinale organoids og retinal pigmenteret epitel til grundlæggende, translationel og klinisk forskning.

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