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Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells

Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells

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10,046 Views

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12:21 min

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March 03, 2016

DOI:

10.3791/53806-v

DOI:
10.3791/53806-v

•

12:21 min
March 03, 2016

•

8 Views
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1The Center for Stem Cell Biology, Developmental Biology Program,Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School,Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

Summary

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This work describes an in vitro differentiation protocol to produce pigmented, mature melanocytes from human pluripotent stem cells via a neural crest and melanoblast intermediate stage using a feeder-free, 25 day protocol.

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Cite this Article

Callahan, S. J., Mica, Y., Studer, L. Feeder-free Derivation of Melanocytes from Human Pluripotent Stem Cells. J. Vis. Exp. (109), e53806, doi:10.3791/53806 (2016).

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