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Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
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发育生物学
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JoVE 杂志 发育生物学
Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

9,111 Views

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11:29 min

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September 06, 2018

DOI:

10.3791/57795-v

DOI:
10.3791/57795-v

•

11:29 min
September 06, 2018

•

4 Views
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1Institut de la Vision,Sorbonne Université, INSERM, CNRS, F-75012 Paris, France

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IPS CellsRetinal Cell ModelsRetinal OrganoidsRetinal Pigmented Epithelial CellsRPE CellsXeno-freeFeeder-freeDifferentiationCulture ConditionsCell IsolationFloating CulturePro B-27 MediumPro N-2 MediumFGF2

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Slembrouck-Brec, A., Nanteau, C., Sahel, J., Goureau, O., Reichman, S. Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models. J. Vis. Exp. (139), e57795, doi:10.3791/57795 (2018).

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