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定义的无异和无饲养培养条件的人 iPSC 视网膜细胞模型的生成

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Developmental Biology

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JoVE Journal Developmental Biology

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

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定义的无异和无饲养培养条件的人 iPSC 视网膜细胞模型的生成

DOI:

10.3791/57795-v

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11:29 min

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September 06, 2018

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Amélie Slembrouck-Brec*¹, Céline Nanteau*¹, José-Alain Sahel, Olivier Goureau, Sacha Reichman

¹Institut de la Vision,Sorbonne Université, INSERM, CNRS, F-75012 Paris, France

Chapters

00:04Title
01:04Generation and Maturation of Retinal Organoids
02:50Generation and Amplification of Human iPSC-derived RPE (hiRPE) Cells
05:17Cryopreservation of Retinal Organoids and hiRPE Cells
07:09Thawing of Retinal Organoids and hiRPE Cells
09:01Results: Generation of All Neural Retina Cell Types and RPE
10:19Conclusion

Summary

Automatic Translation

多潜能干细胞的专业视网膜细胞的产生是干细胞治疗视网膜疾病的一个转折点。本文介绍了一种有效生成视网膜 organoids 和视网膜色素上皮的简单方法, 用于基础、平移和临床研究。

Tags

Keywords: IPS Cells Retinal Cell Models Retinal Organoids Retinal Pigmented Epithelial Cells RPE Cells Xeno-free Feeder-free Differentiation Culture Conditions Cell Isolation Floating Culture Pro B-27 Medium Pro N-2 Medium FGF2

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