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Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
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Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
Capítulos
- 00:04Título
- 01:04Generation and Maturation of Retinal Organoids
- 02:50Generation and Amplification of Human iPSC-derived RPE (hiRPE) Cells
- 05:17Cryopreservation of Retinal Organoids and hiRPE Cells
- 07:09Thawing of Retinal Organoids and hiRPE Cells
- 09:01Results: Generation of All Neural Retina Cell Types and RPE
- 10:19Conclusion
The production of specialized retinal cells from pluripotent stem cells is a turning point in the development of stem cell-based therapy for retinal diseases. The present paper describes a simple method for an efficient generation of retinal organoids and retinal pigmented epithelium for basic, translational, and clinical research.
