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成像 Ca2 +志贺菌感染上皮细胞期间的响应
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells
DOI:

08:56 min

May 24, 2018

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Chapters

  • 00:04Title
  • 00:52Bacterial Preparation
  • 02:30Cell Preparation
  • 04:30Infection and Image Acquisition of Local Ca2 Responses
  • 06:50Results: Calcium Imaging During Shigella Infection
  • 08:21Conclusion

Summary

Automatic Translation

在这里, 我们提出的协议, 可视化钙 (Ca2 +) 的反应引起的 HeLa 细胞感染, 由志贺菌。通过对 ca2 +荧光探针的细菌感染和成像参数进行优化, 提出了由细菌引起的非典型全局和局部 Ca2 +信号在大范围的感染动力学中的特征。

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