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Microscopia di luce-foglio per visualizzazione tridimensionale delle cellule immuni umane
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Immunology and Infection
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JoVE Journal Immunology and Infection
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
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Microscopia di luce-foglio per visualizzazione tridimensionale delle cellule immuni umane

italiano

Automatically Generated

7,960 Views

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09:44 min

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June 13, 2018

DOI:

10.3791/57651-v

DOI:
10.3791/57651-v

•

09:44 min
June 13, 2018

•

4 Views
, , ,
1Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine,Saarland University

Summary

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Qui, presentiamo un protocollo per visualizzare le cellule immunitarie incorporate in una matrice di collagene (3D) tridimensionale utilizzando la microscopia a luce-foglio. Questo protocollo elabora anche come tenere traccia di migrazione cellulare in 3D. Questo protocollo può essere impiegato per altri tipi di cellule in sospensione nella matrice 3D.

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Cite this Article

Schoppmeyer, R., Zhao, R., Hoth, M., Qu, B. Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells. J. Vis. Exp. (136), e57651, doi:10.3791/57651 (2018).

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