Dergi
/
Biyokimya
/
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
JoVE Journal
Biyokimya
Author Produced
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

DOI:
10.3791/57600-v

•

14:32 min

•

April 08, 2018

•

, , , , , , , , ,
1Department of Cell Biology and Biochemistry,Texas Tech University Health Sciences Center, 2Department of Biological Sciences,Texas Tech University, 3CISER (Center for the Integration of STEM Education & Research),Texas Tech University

Bölümler

  • 00:00Başlık
  • 01:24Lysate Preparation
  • 03:51Cytoplamic Lysate Preparation from Cultured Human Cells
  • 05:15Cytoplasmic Lysate Preparation from Mouse Testis
  • 06:53Sucrose Gradient Preparation and Ultracentrifugation
  • 09:21Polysome Fractionation and Sample Collection
  • 11:45Analysis of the Fractions
  • 12:27Sonuçlar
  • 14:14Conclusions

Özet

Otomatik Çeviri

Otomatik Çeviri

The overall goal of polysome profiling technique is analysis of translational activity of individual mRNAs or transcriptome mRNAs during protein synthesis. The method is important for studies of protein synthesis regulation, translation activation and repression in health and multiple human diseases.

Etiketler

Polysome ProfilingMRNA TranslationProtein Synthesis RegulationLeishmaniaHuman CellsMouse TestisSucrose GradientUltracentrifugationRibosome FractionationCytoplasmic LysateCycloheximideCell CulturePBS WashCell Counting

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Ganglioside Extraction, Purification and Profiling

Read Article