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小鼠骨髓源性巨噬细胞 Inflammasome 活化和 Pyroptotic 细胞死亡的检测

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Immunology and Infection

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JoVE Journal Immunology and Infection

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Please note that all translations are automatically generated. Click here for the English version.

小鼠骨髓源性巨噬细胞 Inflammasome 活化和 Pyroptotic 细胞死亡的检测

DOI:

10.3791/57463-v

•

06:52 min

•

May 21, 2018

•

Andreas B. den Hartigh, Susan L. Fink

¹Department of Laboratory Medicine,University of Washington

Chapters

00:04Title
00:45NOD-like RecptorP3 (NLRP3) Inflammasome Activation, Caspase-1 Activity Probe (FAM-YVAD-FMK)-labeling, and Antibody Staining
04:07Lactate Dehydrogenase (LDH) Release Assay
05:23Results: Representative Inflammasome Activation and Pyroptotic Cell Death Detection
06:21Conclusion

Summary

Automatic Translation

我们描述了 NLRP3 inflammasome 激活的细胞基础上的荧光显微镜和染色的主动 caspase-1 和适配器, ASC。以乳酸脱氢酶释放试验为依据, 检测 pyroptotic 裂解的人群。这些技术可以适应研究 inflammasome 生物学的许多方面。

Tags

Inflammasome Activation Pyroptosis Macrophages Fluorescence Microscopy LDH Release Assay Caspase-1 Nigericin FAM-YVAD-FMK Paraformaldehyde Confocal Microscopy

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