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Chapters
- 00:04Title
- 00:40Annealing of vDNA
- 02:35Intasome Assembly
- 04:12Intasome Solubilization and Purification
- 06:23Integration Strand Transfer Assay, Fraction Selection, and Storage
- 08:29Results: Isolation and Evaluation of PFV Intasomes
- 09:45Conclusion
Rekombinant retroviral integrase og DNA oligomers etterligne viral DNA ender kan danne en enzymatisk aktive komplekset kalles en intasome. Intasomes kan brukes for biokjemiske, strukturelle og kinetisk studier. Denne protokollen detaljer kursen og rense prototype skummende virus intasomes.
