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Vergadering en zuivering van Prototype schuimend Virus Intasomes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assembly and Purification of Prototype Foamy Virus Intasomes
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Vergadering en zuivering van Prototype schuimend Virus Intasomes

Nederlands

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5,347 Views

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10:20 min

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March 19, 2018

DOI:

10.3791/57453-v

DOI:
10.3791/57453-v

•

10:20 min
March 19, 2018

•

2 Views
, ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Summary

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Recombinante retrovirale integrase en DNA oligomeren nabootsen van virale DNA uiteinden kunnen een enzymatisch actieve complex bekend als een intasome vormen. Intasomes kan worden gebruikt voor biochemische, structurele en kinetische studies. Dit protocol detailleert hoe te monteren en te zuiveren van prototype schuimend virus intasomes.

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Cite this Article

Mackler, R. M., Lopez Jr., M. A., Yoder, K. E. Assembly and Purification of Prototype Foamy Virus Intasomes. J. Vis. Exp. (133), e57453, doi:10.3791/57453 (2018).

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