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Derleme ve prototip köpüklü virüs Intasomes arıtma
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assembly and Purification of Prototype Foamy Virus Intasomes
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Derleme ve prototip köpüklü virüs Intasomes arıtma

Türkçe

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5,347 Views

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10:20 min

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March 19, 2018

DOI:

10.3791/57453-v

DOI:
10.3791/57453-v

•

10:20 min
March 19, 2018

•

2 Views
, ,
1Department of Cancer Biology and Genetics,The Ohio State University College of Medicine

Summary

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Rekombinant retroviral integrase ve DNA reaksiyonlar viral DNA uçları taklit eden bir intasome bilinen bir enzimatik etkin kompleks oluşabilir. Intasomes biyokimyasal, yapısal ve Kinetik çalışmalar için kullanılır. Bu protokolü nasıl bir araya getiren ve prototip köpüklü virüs intasomes arındırmak ayrıntılı.

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Cite this Article

Mackler, R. M., Lopez Jr., M. A., Yoder, K. E. Assembly and Purification of Prototype Foamy Virus Intasomes. J. Vis. Exp. (133), e57453, doi:10.3791/57453 (2018).

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