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大人の耳の皮膚の共焦点顕微鏡を全取り付ける: 神経血管分岐の形態形成と免疫細胞の分布を検討するモデル システム
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Developmental Biology
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JoVE Journal Developmental Biology
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
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大人の耳の皮膚の共焦点顕微鏡を全取り付ける: 神経血管分岐の形態形成と免疫細胞の分布を検討するモデル システム

日本語

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12,671 Views

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08:42 min

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March 29, 2018

DOI:

10.3791/57406-v

DOI:
10.3791/57406-v

•

08:42 min
March 29, 2018

•

6 Views
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1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

Summary

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ここでは、分岐の形態形成および末梢神経、血管、免疫細胞分布のパターンを視覚化することを可能にする全体の大人マウス耳介皮膚に高解像度全体マウント画像処理手法について述べる。

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Yamazaki, T., Li, W., Mukouyama, Y. Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution. J. Vis. Exp. (133), e57406, doi:10.3791/57406 (2018).

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