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Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

12,718 Views

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08:42 min

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March 29, 2018

DOI:

10.3791/57406-v

DOI:
10.3791/57406-v

•

08:42 min
March 29, 2018

•

6 Views
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1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

Summary

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Here, we describe a high resolution whole-mount imaging method in the entire adult mouse ear skin, which enables us to visualize branching morphogenesis and patterning of peripheral nerves and blood vessels, as well as immune cell distribution.

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Whole-mountConfocal MicroscopyEar SkinNeuro-vascular BranchingMorphogenesisImmune Cell DistributionPeripheral NervesBlood VesselsImmunohistochemistrySkin Preparation3D VisualizationNormal And Pathological Conditions

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Cite this Article

Yamazaki, T., Li, W., Mukouyama, Y. Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution. J. Vis. Exp. (133), e57406, doi:10.3791/57406 (2018).

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