Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Tomoko Yamazaki; Wenling Li; Yoh-Suke Mukouyama

doi:10.3791/57406

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Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

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発生生物学

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JoVE Journal 発生生物学

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

12,690 Views

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08:42 min

•

March 29, 2018

DOI:

10.3791/57406-v

DOI:

10.3791/57406-v

•

08:42 min

March 29, 2018

•

6 Views

Tomoko Yamazaki^1,2, Wenling Li¹, Yoh-Suke Mukouyama¹

¹Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health (NIH), ²Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

概要

Here, we describe a high resolution whole-mount imaging method in the entire adult mouse ear skin, which enables us to visualize branching morphogenesis and patterning of peripheral nerves and blood vessels, as well as immune cell distribution.

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Whole-mount Confocal Microscopy Ear Skin Neuro-vascular Branching Morphogenesis Immune Cell Distribution Peripheral Nerves Blood Vessels Immunohistochemistry Skin Preparation 3D Visualization Normal And Pathological Conditions

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Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

概要

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