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成人耳皮肤全安装共焦显微术: 一种研究神经血管分支形态发生和免疫细胞分布的模型系统
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Developmental Biology
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JoVE Journal Developmental Biology
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
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成人耳皮肤全安装共焦显微术: 一种研究神经血管分支形态发生和免疫细胞分布的模型系统

English

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12,671 Views

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08:42 min

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March 29, 2018

DOI:

10.3791/57406-v

DOI:
10.3791/57406-v

•

08:42 min
March 29, 2018

•

6 Views
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1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

Summary

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在这里, 我们描述了一个高分辨率全安装成像方法在整个成年小鼠耳皮肤, 这使我们能够想象分支形态发生和模式的周围神经和血管, 以及免疫细胞分布。

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Whole-mountConfocal MicroscopyEar SkinNeuro-vascular BranchingMorphogenesisImmune Cell DistributionPeripheral NervesBlood VesselsImmunohistochemistrySkin Preparation3D VisualizationNormal And Pathological Conditions

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Cite this Article

Yamazaki, T., Li, W., Mukouyama, Y. Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution. J. Vis. Exp. (133), e57406, doi:10.3791/57406 (2018).

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