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将套管植入啮齿动物的麦格纳池中
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Cannula Implantation into the Cisterna Magna of Rodents
DOI:

10:13 min

May 23, 2018

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Chapters

  • 00:04Title
  • 01:17Cannula Preparation
  • 01:53Insertion of the Cannula into the Cisterna Magna for Chronic Injections of CSF Tracers
  • 05:52Injection of CSF Tracers
  • 06:43Injecting CSF Tracers in Anesthetized Mice with Chronic Cisterna Magna Cannulation
  • 07:20Injecting CSF Tracers in Awake Mice with Chronic Cisterna Magna Cannulation
  • 08:05Results: Analysis of CSF Tracer Distribution
  • 09:02Conclusion

Summary

Automatic Translation

在这里, 我们描述了一个协议, 以执行麦格纳插管 (CMc), 一种微创的方式, 提供示踪剂, 基质和信号分子进入脑脊液 (CSF)。结合不同的成像方式, CMc 使 glymphatic 系统和脑脊液动力学评估, 以及大脑范围内的各种化合物的交付。

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