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Cannula Implantation into the Cisterna Magna of Rodents
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10:13 min
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May 23, 2018
DOI:
1Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Copenhagen, 2Department of Anesthesiology,Yale School of Medicine, 3Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Rochester Medical Center, 4Department of Experimental Medical Science, Wallenberg Center for Molecular Medicine,Lund University
Summary
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Here we describe a protocol to perform cisterna magna cannulation (CMc), a minimally invasive way to deliver tracers, substrates and signaling molecules into the cerebrospinal fluid (CSF). Combined with different imaging modalities, CMc enables glymphatic system and CSF dynamics assessment, as well as brain-wide delivery of various compounds.
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Xavier, A. L., Hauglund, N. L., von Holstein-Rathlou, S., Li, Q., Sanggaard, S., Lou, N., Lundgaard, I., Nedergaard, M. Cannula Implantation into the Cisterna Magna of Rodents. J. Vis. Exp. (135), e57378, doi:10.3791/57378 (2018).
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